List of publications

Nakamura, M. and Takahashi, H. (1973). M. Nakamura and H. Takahashi , Gonadal sex differentiation in Tilapia mossambica with special regard to the time of estrogen treatment effective in inducing feminization of genetic fishes. Bull. Fac. Fish., Hokkaido Univ. 24: 1-13.

Yamauchi, K., Nakamura, M. ,Takahashi, H and Takano K. (1976). Cultivation of larvae of Japanese eel. Nature. 263 (5576): 412-412.

Nakamura, M. Takahashi, H. and Hiroi, O. (1974). M. Nakamura, H. Takahashi and O. Hiroi , Sex differentiation of the gonad in the Masu salmon (Oncorhynchus masou). Sci. Rep. Hokkaido Salmon Hatchery 28:1?8.

Nakamura M (1975). Dosage-dependent changes in the effect of oral administration of methyltestosterone on gonadal sex differentiation in Tilapia mossambica. Bull. Fac. Fish., Hokkaido Univ. 26: 99?108.

Nakamura, M. (1981). Effect of 11-ketotestosterone on gonadal sex differentiation in Tilapia mossambica. Bull. Fac. Fish., Hokkaido Univ. 47:1323?1327.

Nakamura, M. (1982). Gonadal sex differentiation in whitespotted char, Salvelinus leucomaenis. Jpn. J. Ichthyol. 28: 431?436.

Nakamura, M. and Iwahashi, M. (1982). Studies on the practical masculinization in Tilapia nilotica by the oral administration of androgen. Bull. Jpn. Soc. Sci. Fish. 48 :763?769.

Nakamura, M. (1984). Effects of estradiol-17 beta on gonadal sex differentiation in two species of salmonids, the masu salmon, Oncorhynchus masou, and the chum salmon, O. keta. Aquaculture 43:1-3.

Nakamura M. and Nagahama, Y. (1985). Steroid producing cells during ovarian differentiation of the tilapia, Sarotherodon niloticus. Dev. Growth Differ. 27: 701-708.

Nakamura, M, Nagahama, Y., Iwahashi, M. and Kojima, M. (1987). Ovarian structure and plasma steroid hormones of triploid female rainbow trout. Bull. Jpn. Soc. Sci. Fish./Nippon Suisan Gakkaishi 53:105.

Nakamura, M., Hourigan, T.F., Yamauchi, K. Nagahama, Y. and Grau, E.G. (1989). Histological and ultrastructural evidence for the role of gonadal steroid hormones in sex change in the protogynous wrasse (Thalassoma duperrey). Env. Biol. Fish.. 24: 117-136.

Nakamura, M and Nagahama, Y. (1989). Differentiation and development of Leydig-cells, and changes of testosterone levels during testicular-differentiation in tilapia Oreochromis-niloticus. Fish Physiol Biochem, 7 (1-6): 211-219.

Hourigan, T.F., Nakamura, M., Nagahama, Y., Yamauchi, K. and Grau, E.G. (1991). Histology, ultrastructure, and in vitro steroidogenesis of the testis of 2 male phenotypes of the protogynous fish, Thalassoma-duperrey (Labridae). Gen. Comp. Endocrinol. 83 (2): 193-217.

Nakamura, M. and Nagahama, Y. (1993) Ultrastructural study on the differentiation and development of steroid-producing cells during ovarian differentiation in the amago salmon Oncorhynchus rhodurus. Aquaculture 112 :237?251.

Nakamura, M, Specker, J.L. aand Nagahama, Y. (1993). Ultrastructural analysis of the developing follicle during early vitellogenesis in tilapia, Oreochromis-niloticus, with special reference to the steroid-producing cells. Cell Tissue Res. 272(1): 33-39.

Mita M, Nakamura M. (1993). Lipid globules at the midpieces of Glyptocidaris crenularis spermatozoa and their relation to energy metabolism. Mol Reprod Dev. 34(2):158-63. 

Nakamura, M. (1994). A study of susceptibility of sex reversal after a single 2-hour treatment of androgen in amago salmon. Fish. Sci. 60: 483-484.

Nakamura, M., Mariko T. and Nagahama, Y. (1994). Ultrastructure and in vitro steroidogenesis of the gonads in the protandrous anemonefish Amphiprion frenatus. Jpn. J. Ichthyol. 41: 47-56.

Mita M, Yasumasu I, Nakamura M. (1994). Energy metabolism of spermatozoa of the sand dollar Clypeaster japonicus: the endogenous substrate and ultrastructural correlates. J Biochem (Tokyo). 116(1):108-13.

Mita M, Yasumasu I, Nakamura M. (1995). Endogenous substrate for energy metabolism and ultrastructural correlates in spermatozoa of the sea urchin Diadema setosum. Mol Reprod Dev. 40(1):103-9.

Nakamura, M. Specker, J. and Nagahama, Y. (1996). Innervation of steroid-producing cells in the ovary of tilapia Oreochromis niloticus. Zool. Sci. 13 :603?608.

Chang X.T., Kobayashi T., Kajiura H., Nakamura M., Nagahama Y.(1996. Isolation and characterization of the cDNA encoding the tilapia (Oreochromis niloticus) cytochrome P450 aromatase (P450arom): changes in P450arom mRNA, protein and enzyme activity in ovarian follicles during oogenesis. J Mol. Endocrinol. 18: 57-66

Kobayashi T., Kajiura H., Chang X.T., Nakamura M., Nagahama Y. (1996) Antibodies against trout 3b-hydroxysteroid dehydrogenase: their preparation and immunocytochemical use for fish steroidogenic tissue. Zool Sci 13:909-914.

Chang X.T, Nakamura M., Kobayashi T., Nagahama Y. (1997) Immunohistochemical localization of steroidogenic enzymes in gonads during sex differentiation in tilapia. J Reprod Dev 43 (Suppl): 73-74.

Nakamura M., Kobayashi T, Chang XT, Nagahama, Y. (1998). Gonadal sex differentiation in teleost fish. J. Exp. Zool. 281 (5): 362-372 (Review).

Morrey, C.E., Nakamura, M., Kobayashi, T., Grau, E.G. and Nagahama, Y. (1998). P450scc-like immunoreactivity throughout gonadal restructuring in the protogynous hermaphrodite Thalassoma duperrey. Intl. J. Dev. Biol. 42 (6): 811-816.

Nakamura M. (2000.). Endocrinological studies on sex differentiation and reproduction in fish. Nip. Suisan Gakk. 66 (3): 376-379.

Nakamura M, Uehara T, Mita M. (2000). Ultrastructural study of endogenous energy substrates in spermatozoa of the four species of the sea urchin, Echinometra mataei. Zygote. 8(Suppl 1): 56-7.

Strussmann CA, Nakamura, M. (2002). Morphology, endocrinology, and environmental modulation of gonadal sex differentiation in teleost fishes. Fish Physiol. Biochem. 26 (1): 13-29 (Review)

Kim, S. J., Ogasawara, K., Park, J.G., Takemura, A. and Nakamura, M. (2002). Sequence and expression of androgen receptor and estrogen receptor gene in the sex types of protogynous wrasse, Halichoeres trimaculatus. Gen. Comp. Endocrinol., 127:165-173.

Nakamura, M. Nagoya, H. and Hirai, T. (2002). Nonylphenol induces complete feminization of the gonad in genetically controlled all-male amago salmon. Fisheries Science. 68: 1387 (Short paper).

Mita M, Uehara T, Nakamura M. (2002). Comparative studies on the energy metabolism in spermatozoa of four closely related species of sea urchins (genus Echinometra) in Okinawa. Zoolog Sci.  19(4): 419-27.

Nakamura, M., Bhandari, R.K. and Higa, M. (2003). The role estrogens play in sex differentiation and sex change of fish. Fish Physiol. Biochem. 28: 113-117

Higa, M., Ogasawara, K., Sakaguchi, A., Nagahama, Y. and Nakamura, M. (2003) Role of steroid hormones in sex change of protogynous wrasse. Fish Physiol. Biochem. 28: 149-150.

Miura, S., Komatsu, T., Higa, M., Bhandari, R.K., Nakamura, S. and Nakamura, M. (2003). Gonadal sex differentiation in protandrous anemone fish, Amphiprion clarkii. Fish Physiol. Biochem. 28: 165-166.

Bhandari, R.K., Higa, M., Komuro, H., Nakamura, S. and Nakamura, M. (2003). Treatments with an aromatase inhibitor induces complete sex in protogynous honeycomb grouper (Epinephelus merra). Fish Physiol. Biochem. 28: 141-142.

Bhandari, R.K. Komuro, H., Nakamura, S., Higa, M., and Nakamura, M. (2003).Gonadal restructuring and correlative steroid hormone profiles during natural sex change in protogynous honeycomb grouper, Epinephelus merra. Zool. Sci. 20: 1399-1403.

Bhandari, R.K., Higa, M., Nakamura, S. and Nakamura, M. (2004). Aromatase inhibitor induces complete sex in protogynous honeycomb grouper, Epinephelus merra. Mol. Reprod. Dev. 67 (3): 303-307.

Bhandari R.K., Komuro, H., Higa, M. and Nakamura, M. (2004). Sex inversion of sexually immature female honeycomb grouper by aromatase inhibitor. Zool. Sci. 21(3): 305-310.

Kobayashi Y., Kobayashi T., Nakamura M., Sunobe T., Morrey CE, Suzuki N., and Nagahama Y. (2004). Characterization of two types of cytochrome P450 aromatase in the serial-sex changing gobiid fish, Trimma Okinawae. Zoological Science, 21: 417-425.

Nakamura, M., Higa, M., Ohashi, H., Nagahama, Y. (2004). In vitro spermatogenesis in fish ovary. Biology of Reproduction, 136:124-124.

Bhandari R.K., Alam, M.A., Higa, M., Soyano, K. and Nakamura, M. (2005). Evidence that estrogen regulates the sex change of honeycomb grouper (Epinephelus merra), a protogynous hermaphrodite fish. J. Exp. Zool. (in press)

Alam, M.A., Komuro, H., Bhandari, R.K. , Nakamura, S., Soyano, K., and Nakamura, M., (2005). Immunohistochemical evidence identifying the site of androgen production in the ovary of the protogynous grouper, Epinephelus merra . Cell and Tissue Research (in press)

Bhandari, R.K. , Nakamura, M., Kobayashi,T., and Nagahama, Y. (2005). Suppression of steroidogenic enzyme expression during androgen-induced sex reversal in the Nile tilapia (Oreochromis niloticus) . General and Comparative Endocrinology (in review)

Bhandari, R.K. , Alam, MA, and Nakamura, M., (2005). Efficacy of aromatase inhibitor (Fadrozole) to suppress estradiol-17ƒÀ production in the ovary of honeycomb grouper (Epinephelus merra). Aquaculture (in review)

Conferences and symposiums attended (2003 to date)

Bhandari, R.K., Higa, M. Nakamura, S. and Nakamura, M. Aromatase inhibitor induces complete sex change in protogynous fish, Epinephelus merra. Third International Symposium on vertebrate sex determination. Kona, Hawaii, USA. March 24-28, 2003. pp.96 (Abstract)

Higa, M., Ogasawara, K., Sakaguchi, A., Nagahama, Y. and Nakamura, M. Hormonal regulation of sex change in wrasse. Third International Symposium on vertebrate sex determination. Kona, Hawaii, USA. March 24-28, 2003. pp.8 (Abstract)

Bhandari, R.K., Higa, M., Nakamura, S. and Nakamura, M. Endocrine changes during sex change in a protogynous hermaphrodite fish, Epinephelus merra. 15th Annual meeting of Fisheries Society of Japan. Tokyo University of Fisheries, Tokyo, Japan. April 1-5, 2003.

Nakamura, M. Role of estrogens in sex differentiation and sex change in fish. Seventh International Symposium on Reproductive Biology of Fish. Mie, Japan. May 18-23, 2003.

Bhandari, R.K. Higa, M. Komuro, H. and Nakamura, M. Aromatase inhibitor induces complete sex change in the protogynous honeycomb grouper Epinephelus merra.

Miura, S., Komatsu, T., Higa, M., Bhandari, R.K., Nakamura, S. and Nakamura, M. Gonadal sex differentiation in protandrous anemone fish, Amphiprion clarkii. Seventh International Symposium on Reproductive Biology of Fish. Mie, Japan. May 18-23, 2003.

Higa, M., Ogasawara, K., Sakaguchi, A., Nagahama, Y. and Nakamura, M. Role of steroid hormones in sex change of protogynous wrasse. Seventh International Symposium on Reproductive Biology of Fish. Mie, Japan. May 18-23, 2003.

Nakamura M., Bhandari, R.K. and Higa, M. Functional sex reversal by the treatments of aromatase inhibitor after ovarain differentiation in fish. 74th Annual meeting of Fisheries Society of Japan. Hakodate, Japan. September 17-21, 2003.

Bhandari, R.K., Higa, M. and Nakamura, M. Functional sex reversal after ovarian differentiation in the gonochoristic fish, Nile tilapia: endocrine changes. 74th Annual meeting of Fisheries Society of Japan. Hakodate, Japan. September 17-21, 2003.

Bhandari, R.K., Komuro, H., Nakamura, S., Higa, M., and Nakamura, M. Estrogen depletion causes sex change of an adult hermaphrodite fish. New Horizons in Molecular Sciences and Systems: An Integrated Approach" Okinawa, Japan, October 16-18, 2003 (Submitted).

Bhandari, R.K., Komuro, H. and Higa, M., Nagahama, Y., Nakamura, M. Induction of sex change of female honeycomb grouper, Epinephelus merra, by aromatase inhibitor Fadrozole. Aquaculture 2004. Honolulu Hawaii, March 1-5, 2004.

Bhandari, R.K., Higa, M., Nagahama, Y., and Nakamura, M. Sex reversal of adult gonochoristic fish. 5th International Symposium on Fish Endocrinology, Institute of Aquaculture Torre de la Sal, Spanish Council for Scientific Research (CSIC), Castell?n, Spain, September 5-9, 2004 (Submitted).

Bhandari, R.K. and Nakamura, M. The role of estrogen in protogynous sex change of honeycomb grouper (Epinephelus merra). 5th International Symposium on Fish Endocrinology, Institute of Aquaculture Torre de la Sal, Spanish Council for Scientific Research (CSIC), Castell?n, Spain, September 5-9, 2004 (submitted).